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They also found that PNE increased the expression of the D5 dopamine receptor in the striatum but did not influence exploratory locomotion, the delay-discounting test, or the expression of other dopamine-related genes ( DRD4 , DAT1 , NR4A2 , and TH ) in either the PFC or the striatum [80]

They then used questionnaires to confirm that those customers were current conventional-cigarette smokers and followed up with them after three months